Publications

Transient in vivo cytidine base editing with no detectable RNA- and DNA off-target mutations
L Villiger*, T Rothgangl*, D Witzigmann, R Oka, P J C Lin, W Qi, S Janjuha, C Berk, F Ringnalda, M B Beattie, M Stoffel, B Thöny, J Hall, H Rehrauer, R van Boxtel, Y K Tam and G Schwank
Accepted at Nature Biomedical Engineering, 2020
*These authors contributed equally

Predicting base editing outcomes with an attention-based deep learning algorithm trained on high-throughput target library screens
K F Marquart*, A Allam*, S Janjuha*, A Sintsova, L Villiger, N Frey, M Krauthammer, G Schwank
bioRxiv, 2020
*These authors contributed equally

High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays
N Brandenberg, S Hoehnel, F Kuttler, K Homicsko, C Ceroni, T Ringel, N Gjorevski, G Schwank, G Coukos, G Turcatti, M P Lutolf
Nature Biomedical Engineering, 2020

Genome-Scale CRISPR Screening in Human Intestinal Organoids Identifies Drivers of TGF-β Resistance
T Ringel, N Frey, F Ringnalda, S Janjuha, S Cherkaoui, S Butz, S Srivatsa, M Pirkl, G Russo, L Villiger, G Rogler, H Clevers, N Beerenwinkel, N Zamboni, T Baubec, G Schwank
Cell Stem Cell 26 (3), 431-440. e8, 2020

Germ‐Free and Microbiota‐Associated Mice yield Small Intestinal Epithelial Organoids with Equivalent and Robust Transcriptome/Proteome Expression Phenotypes
A Hausmann, G Russo, J Grossmann, M Zünd, G Schwank, R Aebersold, Y Liu, ME Sellin, W Hardt
Cellular Microbiology, e13191, 2020

State-of-the-art 2019 on gene therapy for phenylketonuria
HM Grisch-Chan, G Schwank, CO Harding, B Thöny
Human Gene Therapy, 30 (10), 1274-1283, 2019

In vitro generation of CRISPR-Cas9 complexes with covalently bound repair templates for genome editing in mammalian cells
N Savić, F Ringnalda, C Berk, K Bargsten, J Hall, M Jinek, G Schwank
Bio-protocol, 9 (1), 2019

Characterization of HIF-dependent alternative isoforms in pancreatic cancer
P Markolin, N Davidson, C Hirt, C Chabbert, N Zamboni, G Schwank, W Krek, G Raetsch
bioRxiv, 826156, 2019

Treatment of a metabolic liver disease by in vivo genome base editing in adult mice
L Villiger, HM Grisch-Chan, H Lindsay, F Ringnalda, CB Pogliano, G Allegri, R Fingerhut, J Häberle, J Matos, MD Robinson, B Thöny, G Schwank
Nature Medicine, 24 (10), 1519-1525, 2018

Growth of epithelial organoids in a defined hydrogel
N Broguiere, L Isenmann, C Hirt, T Ringel, S Placzek, E Cavalli, F Ringnalda, L Villiger, R Züllig, R Lehmann, G Rogler, MH Heim, J Schüler, M Zenobi-Wong, G Schwank
Advanced Materials, 30 (43), 1801621, 2018

Organoid models of human liver cancers derived from tumor needle biopsies
S Nuciforo, I Fofana, MS Matter, T Blumer, D Calabrese, T Boldanova, S Piscuoglio, S Wieland, F Ringnalda, G Schwank, LM Terracciano, CKY Ng, MH Heim
Cell Reports, 24 (5), 1363-1376, 2018

Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair
N Savic, FCAS Ringnalda, H Lindsay, C Berk, K Bargsten, Y Li, D Neri, MD Robinson, C Ciaudo, J Hall, M Jinek, G Schwank
Elife, 7, e33761, 2018

Tissue-specific mutation accumulation in human adult stem cells during life
F Blokzijl, J De Ligt, M Jager, V Sasselli, S Roerink, N Sasaki, M Huch, S Boymans, E Kuijk, P Prins, I Nijman, I Martincorena, M Mokry, C Wiegerinck, S Middendorp, T Sato, G Schwank, E Nieuwenhuis, M Verstegen, L Van Der Laan, J De Jonge, J IJzermans, R Vries, M Van De Wetering, M Stratton, H Clevers, E Cuppen, R Van Boxtel
Nature, 538 (7624), 260-264, 2016

Advances in therapeutic CRISPR/Cas9 genome editing
N Savić, G Schwank
Translational Research, 168, 15-21, 2016

CRISPR/Cas9-mediated genome editing of mouse small intestinal organoids
G Schwank, H Clevers
Gastrointestinal Physiology and Diseases, 3-11, 2016

Sequential cancer mutations in cultured human intestinal stem cells
J Drost, RH Van Jaarsveld, B Ponsioen, C Zimberlin, R Van Boxtel, A Buijs, N Sachs, R Overmeer, G Offerhaus, H Begthel, J Korving, M Van De Wetering, G Schwank, M Logtenberg, E Cuppen, H Snippert, J Medema, G Kops, H Clevers
Nature, 521 (7550), 43-47, 2015

Paneth cell extrusion and release of antimicrobial products is directly controlled by immune cell–derived IFN-γ
HF Farin, WR Karthaus, P Kujala, M Rakhshandehroo, G Schwank, R Vries, E Kalkhoven, E Nieuwenhuis, H Clevers
Journal of Experimental Medicine, 211 (7), 1393-1405, 2014

Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients
G Schwank, BK Koo, V Sasselli, JF Dekkers, I Heo, T Demircan, N Sasaki, S Boymans, E Cuppen, C van der Ent, E Nieuwenhuis, J Beekman, H Clevers
Cell Stem Cell, 13 (6), 653-658, 2013

Generation of BAC transgenic epithelial organoids
G Schwank, A Andersson-Rolf, BK Koo, N Sasaki, H Clevers
PLOS ONE, 8 (10), 2013

Comment on “Dynamics of dpp signaling and proliferation control”
G Schwank, SF Yang, S Restrepo, K Basler
Science, 335 (6067), 401, 2012

Formation of the long range Dpp morphogen gradient
G Schwank, S Dalessi, SF Yang, R Yagi, AM de Lachapelle, M Affolter, S Bergmann, K Basler
PLOS BIOLOGY, 9 (7), 2011

Antagonistic growth regulation by Dpp and Fat drives uniform cell proliferation
G Schwank, G Tauriello, R Yagi, E Kranz, P Koumoutsakos, K Basler
Developmental Cell, 20 (1), 123-130, 2011

Regulation of organ growth by morphogen gradients
G Schwank, K Basler
Cold Spring Harbor Perspectives in Biology, 2 (1), 2010

Growth regulation by Dpp: an essential role for Brinker and a non-essential role for graded signaling levels
G Schwank, S Restrepo, K Basler
Development, 135 (24), 4003-4013, 2018